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Screens

Most of our fish lines are from large-scale screening experiments. As a rule we provide only lines that have been published or submitted to ZFIN. For the correct citation for each allele please refer to ZFIN.

Tübingen I

ENU mutagenesis screen conducted in 1993 - 1996 by the laboratory of Christiane Nüsslein-Volhard at the Max-Planck-Institute for Developmental Biology. Some of the published lines have not yet been sequenced ("unm..." genotypes).

Tübingen 2000

ENU mutagenesis screen conducted in 1999 - 2002 by the laboratory of Christiane Nüsslein-Volhard and Artemis Pharmaceuticals GmbH. Only a small number of lines have been published.

Tübingen EU

ENU mutagenesis screen conducted in 2004 - 2008 by the ZF-MODELS EU consortium in the laboratory of Christiane Nüsslein-Volhard. Most lines were directly submitted to ZFIN and have not yet been sequenced ("unm..." genotypes).

Sanger ...

The ongoing Zebrafish Mutation Project is conducted by Derek Stemple at the Wellcome Trust Sanger Institute, and aims to create knock-outs for all protein-coding genes in the zebrafish by ENU mutagenesis and whole-exome sequencing. Most of these lines have not yet been phenotyped. The library of mutagenized fish is indicated as part of the screen name.

ZETRAP

TOL2-based enhancer-trap insertions generated by the laboratory of Vladimir Korzh at A*STAR, Singapore and described on the ZETRAP 2.0 website.

FISHTRAP

Ds transposon insertions generated by the laboratory of Karuna Sampath at the University of Warwick and described on the FISHTRAP website.

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Fish generations

The indicated generation refers to the fish held by the EZRC. You will receive its progeny, i.e. the next generation.

F1

F1 fish from a screening experiment, generally containing background mutations or transgenic insertions that are not listed in the genotype. A list of known background mutations/insertions will be provided with the shipment.

F2

F2 fish from a screening experiment, still likely to contain some background mutations or transgenic insertions. The actual F2 fish for outcrossing will be selected by EZRC staff based on availability, and may not be IDed for all background mutations. A list of known background mutations/insertions in the F1 and in the selected F2 will be provided with the shipment.

F3 ...

Outcrossed for the indicated number of generations.

outcross

Outcrossed for an undetermined number of generations.

incross

Incrossed for an undetermined number of generations.

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Product categories and pricing

WT embryos

Provided in quantities of 200.

WT adults

Provided in quantities of 5 pairs.

Tg/mut embryos

Provided in quantities of at least 30 (up to 120 depending on fertilization success), generally from an IVF or outcross against AB wild-type females. In some cases an incross may be performed. Information on the type of cross will be provided with the shipment.

Sanger embryos

Provided in quantities of at least 30 (up to 120 depending on fertilization success), from an IVF or outcross against AB wild-type females. If not enough embryos are generated by IVF and not enough sperm samples are available to retry, we will instead grow up the line and ship embryos from identified carriers when they are ready. This may increase shipping time to 4 months or more. Note that we cannot reduce your price in this case.

Tg/mut adults, Sanger adults

Identified adults provided in quantities of 3 pairs.

Plasmid

5 μg of plasmid DNA is provided as a dry spot on a filter paper, to academic customers only.

Additional items are available on request - see EZRC_Pricing_Info.pdf

Pricing

Item (unit size) Academic Commercial
WT embryos (200 embryos) € 150 € 300
WT adults (5 pairs) € 50 *
Tg/mut embryos (30 or more embryos € 300 € 600
Sanger embryos (30 or more embryos) € 550 € 1,100
Tg/mut adults, Sanger adults (3 pairs) € 550 € 1,100
Plasmid (5µg) € 75 n.a.

* NOTE: For-profit organisation shall please contact us for information on availability and prices and to request a quotation.

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Genotype (background)AlleleAffected GenePhenotypeLine TypeScreenGener-
ation
Cate-
gory
Item #
wnt7basa31394 (TL)sa31394wnt7ba-point mutationSanger SL6F1Sanger embryos24854