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Screens

Most of our fish lines are from large-scale screening experiments. As a rule we provide only lines that have been published or submitted to ZFIN. For the correct citation for each allele please refer to ZFIN.

Tübingen I

ENU mutagenesis screen conducted in 1993 - 1996 by the laboratory of Christiane Nüsslein-Volhard at the Max-Planck-Institute for Developmental Biology. Some of the published lines have not yet been sequenced ("unm..." genotypes).

Tübingen 2000

ENU mutagenesis screen conducted in 1999 - 2002 by the laboratory of Christiane Nüsslein-Volhard and Artemis Pharmaceuticals GmbH. Only a small number of lines have been published.

Tübingen EU

ENU mutagenesis screen conducted in 2004 - 2008 by the ZF-MODELS EU consortium in the laboratory of Christiane Nüsslein-Volhard. Most lines were directly submitted to ZFIN and have not yet been sequenced ("unm..." genotypes).

Sanger ...

The ongoing Zebrafish Mutation Project is conducted by Derek Stemple at the Wellcome Trust Sanger Institute, and aims to create knock-outs for all protein-coding genes in the zebrafish by ENU mutagenesis and whole-exome sequencing. Most of these lines have not yet been phenotyped. The library of mutagenized fish is indicated as part of the screen name; we will carry all libraries from SL4 onwards.

ZETRAP

TOL2-based enhancer-trap insertions generated by the laboratory of Vladimir Korzh at A*STAR, Singapore and described on the ZETRAP 2.0 website.

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Fish generations

The indicated generation refers to the fish held by the EZRC. You will receive its progeny, i.e. the next generation.

F1

F1 fish from a screening experiment, generally containing background mutations or transgenic insertions that are not listed in the genotype. A list of known background mutations/insertions will be provided with the shipment.

F2

F2 fish from a screening experiment, still likely to contain some background mutations or transgenic insertions. The actual F2 fish for outcrossing will be selected by EZRC staff based on availability, and may not be IDed for all background mutations. A list of known background mutations/insertions in the F1 and in the selected F2 will be provided with the shipment.

F3 ...

Outcrossed for the indicated number of generations.

outcross

Outcrossed for an undetermined number of generations.

incross

Incrossed for an undetermined number of generations.

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Product categories and pricing

We ship zebrafish only as bleached embryos.

Wild-type

Embryos are provided in quantities of 100, always from an incross.

Living

Embryos are provided in quantities of 30, either from an incross or an outcross against AB wild-type based on availability of females. Information on the type of cross will be provided with the shipment. Please note that we may freeze this line at any time, so order soon!

Living*

These lines are currently being grown up. We will ship embryos when carriers are ready, which may increase shipping time to 6 months or more. Please note that we may freeze this line as soon as carriers are available, so order soon!

Frozen

We attempt to provide embryos in quantities of 30, from in vitro fertilization of AB eggs with frozen sperm. However, if not enough embryos are generated and not enough sperm samples are available to retry, we will instead grow up the line and ship embryos when carriers are ready. This may increase shipping time to 9 months or more. Note that we cannot reduce your price in this case.

Plasmid

5 μg of plasmid DNA is provided as a dry spot on a filter paper, to academic customers only.

Pricing

Item (unit size) Academic Commercial
Wild-type (100 embryos) € 50 € 100
Living, Living* (30 or more embryos) € 125 € 250
Frozen (30 or more embryos) € 450 € 900
Plasmid (5μg) € 50 n.a.
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Line 1 - 1 of 1        Sort by:     
Genotype (background)AlleleAffected GenePhenotypeLine TypeScreenGener-
ation
Cate-
gory
Item #
man1a2sa15063 (TL)sa15063man1a2-point mutationSanger SL4F2frozen14339

Note: We usually ship within 2 months but may take up to 9 months for Sanger F1 lines if IVF yield is insufficient for direct shipment.


Catalog last updated: September 20, 2017