Screening service

Genetic and chemical screens

EZRC will organize collaborative screens where KIT will provide the facilities (including our high-throughput screening pipeline), biological material, software and coordination, while guests bring their own assays. We expect to screen mainly TILLING mutants which will be continuously supplied by the Sanger Institute (approx. 4 families representing a total of 60 KOs per week).

We also offer chemical screening in specific zebrafish lines. Screens can be performed by guest researchers themselves or as a service.

Resources of the screening facility

We have established a pipeline for high-throughput, high-content studies that includes:

• Automated embryo sorters
• Automated imaging systems for behavioral studies
• Robotic microscopes (Olympus Scan^R, enabled for automated and high resolution screening)
• Digital light sheet microscopy (DSLM).

The Complat platform of IBCS-FMS can provide custom chemical libraries for optimization of lead compounds. In addition several standard libraries are available in-house.

Further information is available at the Screening Center website.